黄芩中类黄酮的HPLC-UV色谱图(a)及总离子流图(b)

l-Chrysin-7-O-glu-8-C-glu; 2-Apigenin-6-C-glu-8-C-ara（夏佛托苷）；3-Chrysin-6, 8-di-C-glu;4    Chrysin-6-C-ara-8-C-glu; 5-Isorhamnetin-7-O-rha-glu; 6-Chrysin-6-C-glu-8-C-ara; 7-Wogonin-7-O-glu-glu acid;  8—3’，4’一Dihydroxy-5, 6-dimethoxy-7-O-glucoside flavone; 9-1sorhamnetin-7-O-rha-glu acid;10-5,2'-Dihydroxy-6'-methoxy-7-O-gLu acid flavone;  ll-Baicalin;  12-Baicalein-7-O-glu;  13-5, 6-D1-hydroxy-7-O-glu acid flavanone; 14-Galengin-7-O-glu acid; 15-Wogonin-5-O-glu; 16-Oroxylin A-7-O glu acid; 17-Wogonoside; 18-5,7,3-Trihydroxy-4'-methoxy flavone; 19-Norwogonin; 20-Baicalein;21-5,7 ,4'-Trihydroxy-8-methoxy flavone; 22-5, 2'-Dihydroxy-7,8,6'-trimethoxy  flavone(Skullcapflavon I); 23-Wogonin; 24-5,2'-Dihydroxy-6,7,8,6'-tetramethoxy flavone (Skullcapflavon 11);  25-5,7-Dihydroxy-6-methoxy flavone(Oroxylin A); 26-5,2'-Dihydroxy-6,7,8-trimethoxy flavone (Tenaxin I)

色谱柱：ZorbaxXDB-C18·5μm, 250mmX4.6mmI.D．
流动相：A．乙腈；B．水（0.2%乙酸）
梯度条件：O-lOmin,20%A;lo-llmin,20%-25%A;ll-25min,25%A;25-26min,25%-35%A;26-
        41min,35%A;41—45min,35%--55%A;45-55min,55%-60%A;55—60min,60%A
柱温：25℃
流速：0.8mL/min
检测器：DAD,280nm;ESI
进样量：10μL