Catechins(儿茶素）

色谱峰：Sample:1; Gallocatechin(GC)(0.80mg/ml) 2; Caffeine(0.08mg/ml) 3; Epigallocatechin(EGC)(0.80mg/ml) 4; Catechin (C)(0.40mg/ml) 5; Epicatechin(EC)(0.40mg/ml) 6; Epigallocatechin gallate(EGCG)(0.20mg/ml) 7; Gallocatechin gallate(GCG)(0.40mg/ml) 8; Epicatechin gallate(ECG)(0.20mg/ml) 9; Catechin gallate(CG)(0.20mg/ml)Injection Vol. 1.0氀

色谱条件：Column:Columnsize:4.6mmI.D.-150mmMobilephase:A:Acetonitrile/20mmol/lPhosphatebuffer(pH2.5)=10/90B:Acetonitrile/20mmol/lPhosphatebuffer(pH2.5)=30/70Bconc.0100%20minLineargradientFlowrate:1.0ml/minTemperature:30°CDetection:UV280nm

检测器：UV（紫外光检测器）

色谱柱COSMOSIL Cholester

色谱柱规格250mm * 4.6mm * 5.0μm

色谱柱厂家苏州麦可旺志生物技术有限公司

仪器厂家 / 仪器名称：Agilent 1200 Infinity 系列液相色谱

参考资料：Preparative Separation of cis- and trans-Isomersof nsaturated Fatty Acid Methyl Esters Contained in Edible Oils by Reversed-Phase High- Performance Liquid Chromatography.Tsuzuki W, Ushida K. Lipids 2009 44(4) 373-379