荧光定量PCR原理

荧光定量PCR原理
•TaqMan 探针法
•SYBR Green I 染料法

Polymerization



Displacement


Cleavage


SYBR® Green 1 染料法
SYBR® Green 1 ---Double Stranded DNA Binding Dye Assays



TaqMan探针法定量的特点
􀂾High specificity--- primer and probe based
􀂾high accuracy
􀂾Multiple Reporter---Mutation Detection

SYBR® Green 1 染料法定量的特点
Primer-based specificity
• Target identification (screening) assays
• Convenient than Taqman
• Low-cost assays where high specificity is not required
• Can not do mutation Detection